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3.27. What Is the Difference Between Z Step and µm/pixel (Z) in the Properties?

What Is the Difference Between Z Step and µm/pixel (Z) in the Properties?

• Z Step (µm):

  • Refers to the physical step used to capture the original images.
  • Changing this value does not affect how the data is represented in the image—it is purely for reference purposes.

• µm/pixel (Z):

  • Refers to the Z calibration, which is the optical step corrected for spherical aberration.
  • This value may be the same as the physical step but can be adjusted to account for spherical aberration, providing more accurate calibration.

Understanding these differences ensures accurate representation and interpretation of your imaging data.