Home → Volocity → General → Why Do My Objects Appear Elongated in the Z Axis?
3.32. Why Do My Objects Appear Elongated in the Z Axis?
Why Do My Objects Appear Elongated in the Z Axis?
Elongation in the Z axis is a common issue in optical microscopy and is often caused by a mismatch between the physical and optical step size between Z layers during imaging.
Steps to Address Z-Axis Elongation:
-
Verify Spatial Calibration:
- Ensure that the spatial calibration of the objective lenses has been performed correctly.
- Detailed instructions are available in the Volocity User Guide (pages 120-121).
-
Understand Optical Aberrations:
- Elongation in Z can result from axial aberrations inherent to optical microscopy.
- These factors are discussed in the Volocity User Guide (pages 355-356) and in Technical Note 382.
Additional References on Z-Axis Aberrations:
- Kam, Z. et al. (2001). Computational adaptive optics for live three-dimensional biological imaging. PNAS, 98, 3790-3795.
- Scalettar, B. A. et al. (1996). Dispersion, aberration, and deconvolution in multi-wavelength fluorescence images. Journal of Microscopy, 182, 50-60.
- Sherman, J. et al. (2002). Adaptive correction of depth-induced aberrations in multiphoton scanning microscopy using a deformable mirror. Journal of Microscopy, 206, 65-71.
This page was: Helpful |
Not Helpful